plasmid d135s mutant protein Search Results


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KEY RESOURCES TABLE
Bl21 Bacteria, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Journal: Molecular cell

Article Title: Mettl1/Wdr4-mediated m 7 G tRNA methylome is required for normal mRNA translation and embryonic stem cell self-renewal and differentiation

doi: 10.1016/j.molcel.2018.06.001

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Recombinant wild-type and D135S AlkB proteins were purified as previous described ( Lin and Gregory, 2015 ). pET30a-AlkB and pET30a-AlkB-D135S were gifts from Tao Pan (Addgene plasmid # 79050 and #79051)( Zheng et al., 2015 ). pET30a-AlkB and pET30a-AlkB-D135S were transformed into BL21 bacteria for induced expression of recombinant proteins.

Techniques: Virus, Recombinant, Transfection, Reverse Transcription, Sample Prep, Mutagenesis, SYBR Green Assay, Isolation, Purification, Staining, Plasmid Preparation, Software